An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples

Margaret G. Mills, Emily Bruce, Meei-Li Huang, Jessica W. Crothers, Ollivier Hyrien, Christopher A. L. Oura, Lemar Blake, Arianne Brown Jordan, Susan Hester, Leah Wehmas, Bernard Mari, Pascal Barby, Caroline Lacoux, Julien Fassy, Pablo Vial, Cecilia Vial, Jose R.W. Martinez, Olusola Olalekan Oladipo, Bitrus Inuwa, Ismaila Shittu, Clement A. Meseko, Roger Chammas, Carlos Ferreira Santos, Thiago José Dionísio, Thais Francini Garbieri, Viviane Aparecida Parisi, Maria Cassia Mendes-Correa, Anderson V. dePaula, Camila M. Romano, Luiz Gustavo Bentim Góes, Paola Minoprio, Angelica C. Campos, Marielton P. Cunha, Ana Paula P. Vilela, Tonney Nyirenda, Rajhab Sawasawa Mkakosya, Adamson S. Muula, Rebekah E. Dumm, Rebecca M. Harris, Constance A. Mitchell, Syril Pettit, Jason Botten, Keith R. Jerome

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DOI: 10.1101/2021.04.10.21254091
Published: 2021-04-14
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Publisher: openRxiv
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Cite this work

BibTeX

@article{allodium:10.1101/2021.04.10.21254091,
  title = {An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples},
  author = {Margaret G. Mills and Emily Bruce and Meei-Li Huang and Jessica W. Crothers and Ollivier Hyrien and Christopher A. L. Oura and Lemar Blake and Arianne Brown Jordan and Susan Hester and Leah Wehmas and Bernard Mari and Pascal Barby and Caroline Lacoux and Julien Fassy and Pablo Vial and Cecilia Vial and Jose R.W. Martinez and Olusola Olalekan Oladipo and Bitrus Inuwa and Ismaila Shittu and Clement A. Meseko and Roger Chammas and Carlos Ferreira Santos and Thiago José Dionísio and Thais Francini Garbieri and Viviane Aparecida Parisi and Maria Cassia Mendes-Correa and Anderson V. dePaula and Camila M. Romano and Luiz Gustavo Bentim Góes and Paola Minoprio and Angelica C. Campos and Marielton P. Cunha and Ana Paula P. Vilela and Tonney Nyirenda and Rajhab Sawasawa Mkakosya and Adamson S. Muula and Rebekah E. Dumm and Rebecca M. Harris and Constance A. Mitchell and Syril Pettit and Jason Botten and Keith R. Jerome},
  year = {2021},
  doi = {10.1101/2021.04.10.21254091},
  url = {https://doi.org/10.1101/2021.04.10.21254091}
}

RIS

TY  - JOUR
TI  - An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples
AU  - Margaret G. Mills
AU  - Emily Bruce
AU  - Meei-Li Huang
AU  - Jessica W. Crothers
AU  - Ollivier Hyrien
AU  - Christopher A. L. Oura
AU  - Lemar Blake
AU  - Arianne Brown Jordan
AU  - Susan Hester
AU  - Leah Wehmas
AU  - Bernard Mari
AU  - Pascal Barby
AU  - Caroline Lacoux
AU  - Julien Fassy
AU  - Pablo Vial
AU  - Cecilia Vial
AU  - Jose R.W. Martinez
AU  - Olusola Olalekan Oladipo
AU  - Bitrus Inuwa
AU  - Ismaila Shittu
AU  - Clement A. Meseko
AU  - Roger Chammas
AU  - Carlos Ferreira Santos
AU  - Thiago José Dionísio
AU  - Thais Francini Garbieri
AU  - Viviane Aparecida Parisi
AU  - Maria Cassia Mendes-Correa
AU  - Anderson V. dePaula
AU  - Camila M. Romano
AU  - Luiz Gustavo Bentim Góes
AU  - Paola Minoprio
AU  - Angelica C. Campos
AU  - Marielton P. Cunha
AU  - Ana Paula P. Vilela
AU  - Tonney Nyirenda
AU  - Rajhab Sawasawa Mkakosya
AU  - Adamson S. Muula
AU  - Rebekah E. Dumm
AU  - Rebecca M. Harris
AU  - Constance A. Mitchell
AU  - Syril Pettit
AU  - Jason Botten
AU  - Keith R. Jerome
PY  - 2021
DO  - 10.1101/2021.04.10.21254091
UR  - https://doi.org/10.1101/2021.04.10.21254091
ER  -

APA

Mills, M. G., Bruce, E., Huang, M., Crothers, J. W., Hyrien, O., Oura, C. A. L., Blake, L., Jordan, A. B., Hester, S., Wehmas, L., Mari, B., Barby, P., Lacoux, C., Fassy, J., Vial, P., Vial, C., Martinez, J. R., Oladipo, O. O., Inuwa, B., Shittu, I., Meseko, C. A., Chammas, R., Santos, C. F., Dionísio, T. J., Garbieri, T. F., Parisi, V. A., Mendes-Correa, M. C., dePaula, A. V., Romano, C. M., Góes, L. G. B., Minoprio, P., Campos, A. C., Cunha, M. P., Vilela, A. P. P., Nyirenda, T., Mkakosya, R. S., Muula, A. S., Dumm, R. E., Harris, R. M., Mitchell, C. A., Pettit, S., Botten, J., & Jerome, K. R. (2021). An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples. https://doi.org/10.1101/2021.04.10.21254091

Source records

crossref · retrieved 2026-09-26T04:58:42.681Z